Need advice to make outgoing mail work again

This has gotten too weird for me to figure out, and I'm looking for some help. It's like Mail has decided to stop sending mail. I'll explain the problem and then I can explain why it's almost certainly a problem on my end.
Any mail I send gives me error messages.
If I use my company's server, I get the message:
Cannot send message using the server mail.mydomain.us
The server response was: sorry, that domain isn't allowed to be relayed thru this MTA (#5.7.1)
Use the pop-up menu below to try a different outgoing mail server. All messages will use this server until you quit Mail or change your network settings.
If I try to use my Roadrunner server, I get
Cannot send message using the server smtp-server.houston.rr.com
Use the pop-up menu ...
If my wife sends mail using Mail from her machine, using our company server, it goes through.
I can receive mail via both mail servers, no problem.
If I go to the web interface for my server, I can send and receive.
The last note I was able to send (from my domain) was Sunday night at 10:16 pm. The first message I tried to send the next morning, I got this error. No Mail settings were changed. No network settings were changed -- I know, that makes it sounds like it can't be a Mail problem, but I don't know what other choices I have.
I've logged off and back on with no change. I've rebooted the machine with no change.

Are you also unable to send messages via your RR email account using RR's SMTP server?
Is your company's SMTP server authenticated?
Are you using 25 for the Server Port with your company's SMTP server and if so, have you tried changing the server port from 25 to 587?
FYI - sending messages via webmail access using a browser is not done with an SMTP server as is required when sending messages with an email client such as the Mail application so this is not related.
Do you have any SMTP servers available with Mail that are not being actively used by any email account?
I am also a RR customer and I use RR's SMTP server to send messages with my .Mac account when connected to the internet at home via RR with no problems.

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Need advice to make outgoing mail work again

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