Need help with info about the possibility of altering images when saved in a different format

I am very new to the design world.  I have a printing business and received and image to be printed in Adobe Acrobat (I think).  I needed to resize the image to be printed and took it into Photoshop CS6 where I cropped the edges.  I then resave it as a PDF and took into a printer software, Fiery.  Fiery has an Impose function where you can repeat an image to layout it on the correct paper size.  It essentially just copies the original image several times depending on the layout.  This particular image I repeated to 3 up and 1 across.  When printed it came out with a magenta streak through the center image only.  I have had the machine checked and they are adamant it's not a printer issue but a software issue.  That once I altered the original image by resizing it that I altered the image and that I can't expect it to come out as seen on the screen.  I've printed it all sorts of different ways and it only occurs with the 3 up 1 across image.  I know that images can be changed when saved from program to program but this situation does not make sense that it is only the one area and layout that this shows up. I would appreciate any help, input, answers that anyone has.  Again, I'm new to this and would like to understand if this is a potential for further printing jobs so that I can do what I need to avoid this situation. 
Thank you for your time.
Jessica

Jessica,
Without knowing how the original graphic was created it is hard to tell you how to handle it. What is the name of the file? (Only the file suffix is important). If the file's origin was in a drawing program and/Or InDesign, then the document should be handled differently if the document is a raster image best handled by a program like Photoshop.

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