No more connections available to this remote computer...Urgent Help for File server...

Question

Hi Guys,
I need urgent help regards to our school File server which is having "No more connection to this remote computer error"
on SMB Shares where I usually authenticate with a domain username it used to work fine since till 3 weeks ago.
Now I can browse the SMB shares with only IP... Netbios gets straight away this error message from non-domain joined pc's where the computer needs to connect to the SCCM distribution point on this file server.
Strangely, Domain joined pc's does not have this problem at all they can use Netbios or IP no problem at all. But someone from a standalone laptop or desktop who needs to access shares through netbios gets this message.
Can someone help me please?
I did check DNS yet there is no problem on DNS I checked PTR record for the server to make sure it is not turned in to multihome accidently but nothing at all.
I also did a NETMON where I captured the data from server and the client I get these errors:
205 4:54:35 PM 16/12/2013
7.3299179 10.2.1.96
10.2.0.13 TCP
TCP:Flags=......S., SrcPort=49312, DstPort=Microsoft-DS(445), PayloadLen=0, Seq=1771710719, Ack=0, Win=8192 ( Negotiating scale factor 0x2 ) = 8192
{TCP:22, IPv4:21}
206 4:54:35 PM 16/12/2013
7.3299668 10.2.0.13
10.2.1.96 TCP
TCP:Flags=...A..S., SrcPort=Microsoft-DS(445), DstPort=49312, PayloadLen=0, Seq=2167618331, Ack=1771710720, Win=8192 ( Negotiated scale factor 0x8 ) = 2097152
{TCP:22, IPv4:21}
207 4:54:35 PM 16/12/2013
7.3301468 10.2.1.96
10.2.0.13 TCP
TCP:Flags=...A...., SrcPort=49312, DstPort=Microsoft-DS(445), PayloadLen=0, Seq=1771710720, Ack=2167618332, Win=16425 (scale factor 0x2) = 65700
{TCP:22, IPv4:21}
208 4:54:35 PM 16/12/2013
7.3301468 10.2.1.96
10.2.0.13 SMB
SMB:C; Negotiate, Dialect = PC NETWORK PROGRAM 1.0, LANMAN1.0, Windows for Workgroups 3.1a, LM1.2X002, LANMAN2.1, NT LM 0.12, SMB 2.002, SMB 2.???
{SMBOverTCP:23, TCP:22, IPv4:21}
209 4:54:35 PM 16/12/2013
7.3307974 10.2.0.13
10.2.1.96 SMB2
SMB2:R   NEGOTIATE (0x0), GUID={8447F237-5219-D48A-40C0-29092450C68E}
{SMBOverTCP:23, TCP:22, IPv4:21}
210 4:54:35 PM 16/12/2013
7.3314064 10.2.1.96
10.2.0.13 SMB2
SMB2:C   SESSION SETUP (0x1) {SMBOverTCP:23, TCP:22, IPv4:21}
211 4:54:35 PM 16/12/2013
7.3318815 10.2.0.13
10.2.1.96 SMB2
SMB2:R  - NT Status: System - Error, Code = (22) STATUS_MORE_PROCESSING_REQUIRED  SESSION SETUP (0x1), SessionFlags=0x0
{SMBOverTCP:23, TCP:22, IPv4:21}
212 4:54:35 PM 16/12/2013
7.3324012 10.2.1.96
10.2.0.13 SMB2
SMB2:C   SESSION SETUP (0x1) {SMBOverTCP:23, TCP:22, IPv4:21}
215 4:54:35 PM 16/12/2013
7.3339977 10.2.0.13
10.2.1.96 SMB2
SMB2:R  - NT Status: System - Error, Code = (109) STATUS_LOGON_FAILURE  SESSION SETUP (0x1)  
{SMBOverTCP:23, TCP:22, IPv4:21}
216 4:54:35 PM 16/12/2013
7.3341805 10.2.1.96
10.2.0.13 TCP
TCP:Flags=...A.R.., SrcPort=49312, DstPort=Microsoft-DS(445), PayloadLen=0, Seq=1771711788, Ack=2167619070, Win=0 (scale factor 0x2) = 0
{TCP:22, IPv4:21}
217 4:54:35 PM 16/12/2013
7.3357089 10.2.1.96
10.2.0.13 TCP
TCP:Flags=......S., SrcPort=49313, DstPort=Microsoft-DS(445), PayloadLen=0, Seq=2943201172, Ack=0, Win=8192 ( Negotiating scale factor 0x2 ) = 8192
{TCP:27, IPv4:21}
218 4:54:35 PM 16/12/2013
7.3357422 10.2.0.13
10.2.1.96 TCP
TCP:Flags=...A..S., SrcPort=Microsoft-DS(445), DstPort=49313, PayloadLen=0, Seq=3718656547, Ack=2943201173, Win=8192 ( Negotiated scale factor 0x8 ) = 2097152
{TCP:27, IPv4:21}
219 4:54:35 PM 16/12/2013
7.3359768 10.2.1.96
10.2.0.13 TCP
TCP:Flags=...A...., SrcPort=49313, DstPort=Microsoft-DS(445), PayloadLen=0, Seq=2943201173, Ack=3718656548, Win=16425 (scale factor 0x2) = 65700
{TCP:27, IPv4:21}
220 4:54:35 PM 16/12/2013
7.3359768 10.2.1.96
10.2.0.13 SMB2
SMB2:C   NEGOTIATE (0x0), GUID={8213462D-2600-D1B1-11E3-65FC4BCDE707}
{SMBOverTCP:28, TCP:27, IPv4:21}
221 4:54:35 PM 16/12/2013
7.3364173 10.2.0.13
10.2.1.96 SMB2
SMB2:R   NEGOTIATE (0x0), GUID={8447F237-5219-D48A-40C0-29092450C68E}
{SMBOverTCP:28, TCP:27, IPv4:21}
222 4:54:35 PM 16/12/2013
7.3369702 10.2.1.96
10.2.0.13 SMB2
SMB2:C   SESSION SETUP (0x1) {SMBOverTCP:28, TCP:27, IPv4:21}
223 4:54:35 PM 16/12/2013
7.3373474 10.2.0.13
10.2.1.96 SMB2
SMB2:R  - NT Status: System - Error, Code = (22) STATUS_MORE_PROCESSING_REQUIRED  SESSION SETUP (0x1), SessionFlags=0x0
{SMBOverTCP:28, TCP:27, IPv4:21}
224 4:54:35 PM 16/12/2013
7.3377060 10.2.1.96
10.2.0.13 SMB2
SMB2:C   SESSION SETUP (0x1) {SMBOverTCP:28, TCP:27, IPv4:21}
227 4:54:35 PM 16/12/2013
7.3390552 10.2.0.13
10.2.1.96 SMB2
SMB2:R  - NT Status: System - Error, Code = (109) STATUS_LOGON_FAILURE  SESSION SETUP (0x1)  
{SMBOverTCP:28, TCP:27, IPv4:21}
Regards,
Gokhan

Answer 1

Solution has been found, 
After doing bit of a backtrack, site DC's were out of sync with PDC with time.
Also Time service was shutdown on PDC which has been enabled and pointed to the Australian pool ntp IP address.
Everything is back on track now.
Regards,
Gokhan Cil