Only use two colours in one video?

Hello,
Im looking for help with one of my video.
Im new to after effect, just really know the basics, I've tried looking on utube etc for help but cant fine any.
I have a video where I only want to use 2 colours, I know how to set it up using only one colour, if I try using the same technique for the 2nd colour on the same video, it doesn't work.
How can I get it so it will only use 2 colours?
The colours are red and blue.
thanks

Hello,
Sorry, I will give you a example -
Adobe Premiere Pro - Sin City Effect -- The Rain & The Roses - YouTube
Its back and only the rose is red, this is what I want to do, but instead of using only the red, I would like to highlight a couple of things in blue in my own video.
So, if you take the above video, at the time 2.27 to 2.30, where you see the rose red, how would you get the leaves which are below the rose blue?
I already know how to do the black and red effect.

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