Premiere CS 5 playback stops

I have a dilemma, can anyone help please?
I have Premiere CS 5.0.3 running on a year or two old Mac pro on OS 10.6.6. Just recently I've had the playback stop after a second or less no matter where in the timeline or which project I open. Playback just doesn't work. The problem started after we ran an Adobe update; coincidence or no. We upgraded the RAM to 16gig, we moved the capture footage on and off the same drive to see if that was the problem. I've even tried to searching out the com.apple.audio.AggregateDevices.plist file to delete but it doesn't seem to exist on my computer.
Also, the video card is 32bit.
What could the issue be? I'm trying not to reinstall Premiere because that's my last straw.
Any help would be much appreciated.
Thanks

Welcome to the forum.
Can you give us a bit more to work with?
The Sequence Preset, and the full details of the source footage would be great.
The specs, and especially the CPU and I/O sub-system configuration would be useful too.
This ARTICLE will give you some tips on what might hold the key.
Good luck,
Hunt

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    # for this location.
    #   EncryptionScope server
    #   ProtectionScheme phds
    </Location>
    </IfModule>
    TypesConfig conf/mime.types
    MIMEMagicFile conf/magic
    DefaultType text/plain
    AddType video/x-flv .flv
    AddType video/MP2T .ts
    AddType application/x-compress .Z
    AddType application/x-gzip .gz .tgz
    AddType application/x-mpegurl .m3u8
    AddHandler send-as-is asis
    AddHandler type-map var
    AddType text/html .shtml
    AddOutputFilter INCLUDES .shtml
    <IfModule ssl_module>
        SSLRandomSeed startup builtin
        SSLRandomSeed connect builtin
    </IfModule>
    <IfModule userdir_module>
        UserDir disable
    </IfModule>
    <IfModule dav_fs_module>
        DAVLockDB tmp/dav/lockdb
    </IfModule>
    IndexOptions FancyIndexing VersionSort NameWidth=* HTMLTable
    AddIconByEncoding (CMP,/icons/compressed.gif) x-compress x-gzip
    AddIconByType (TXT,/icons/text.gif) text/*
    AddIconByType (IMG,/icons/image2.gif) image/*
    AddIconByType (SND,/icons/sound2.gif) audio/*
    AddIconByType (VID,/icons/movie.gif) video/*
    AddIconByType (VID,/icons/movie.gif) application/x-shockwave-flash
    AddIcon /icons/binary.gif .bin .exe
    AddIcon /icons/binhex.gif .hqx
    AddIcon /icons/tar.gif .tar
    AddIcon /icons/world2.gif .wrl .wrl.gz .vrml .vrm .iv
    AddIcon /icons/compressed.gif .Z .z .tgz .gz .zip
    AddIcon /icons/a.gif .ps .ai .eps
    AddIcon /icons/layout.gif .html .shtml .htm .pdf
    AddIcon /icons/text.gif .txt
    AddIcon /icons/c.gif .c
    AddIcon /icons/p.gif .pl .py
    AddIcon /icons/f.gif .for
    AddIcon /icons/dvi.gif .dvi
    AddIcon /icons/uuencoded.gif .uu
    AddIcon /icons/script.gif .conf .sh .shar .csh .ksh .tcl
    AddIcon /icons/tex.gif .tex
    AddIcon /icons/bomb.gif core
    AddIcon /icons/back.gif ..
    AddIcon /icons/hand.right.gif README
    AddIcon /icons/folder.gif ^^DIRECTORY^^
    AddIcon /icons/blank.gif ^^BLANKICON^^
    DefaultIcon /icons/unknown.gif
    ReadmeName README.html
    HeaderName HEADER.html
    IndexIgnore .??* *~ *# HEADER* README* RCS CVS *,v *,t
    AddLanguage ca .ca
    AddLanguage cs .cz .cs
    AddLanguage da .dk
    AddLanguage de .de
    AddLanguage el .el
    AddLanguage en .en
    AddLanguage eo .eo
    AddLanguage es .es
    AddLanguage et .et
    AddLanguage fr .fr
    AddLanguage he .he
    AddLanguage hr .hr
    AddLanguage it .it
    AddLanguage ja .ja
    AddLanguage ko .ko
    AddLanguage ltz .ltz
    AddLanguage nl .nl
    AddLanguage nn .nn
    AddLanguage no .no
    AddLanguage pl .po
    AddLanguage pt .pt
    AddLanguage pt-BR .pt-br
    AddLanguage ru .ru
    AddLanguage sv .sv
    AddLanguage zh-CN .zh-cn
    AddLanguage zh-TW .zh-tw
    LanguagePriority en ca cs da de el eo es et fr he hr it ja ko ltz nl nn no pl pt pt-BR ru sv zh-CN zh-TW
    ForceLanguagePriority Prefer Fallback
    AddDefaultCharset UTF-8
    <IfModule mod_negotiation.c>
        <IfModule mod_include.c>
            <Directory "error">
                AllowOverride None
                Options IncludesNoExec
                AddOutputFilter Includes html
                AddHandler type-map var
                Order allow,deny
                Allow from all
                LanguagePriority en es de fr
                ForceLanguagePriority Prefer Fallback
            </Directory>
        </IfModule>
    </IfModule>
    BrowserMatch "Mozilla/2" nokeepalive
    BrowserMatch "MSIE 4\.0b2;" nokeepalive downgrade-1.0 force-response-1.0
    BrowserMatch "RealPlayer 4\.0" force-response-1.0
    BrowserMatch "Java/1\.0" force-response-1.0
    BrowserMatch "JDK/1\.0" force-response-1.0
    BrowserMatch "Microsoft Data Access Internet Publishing Provider" redirect-carefully
    BrowserMatch "MS FrontPage" redirect-carefully
    BrowserMatch "^WebDrive" redirect-carefully
    BrowserMatch "^WebDAVFS/1.[0123]" redirect-carefully
    BrowserMatch "^gnome-vfs/1.0" redirect-carefully
    BrowserMatch "^XML Spy" redirect-carefully
    BrowserMatch "^Dreamweaver-WebDAV-SCM1" redirect-carefully
    #Uncomment following for enabling https based key serving
    #for HLS.
    #Include conf/httpd-hls-secure.conf

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