Premiere cs4 exporting audio problem when I use desktop editing mode

Hi.... I recently use a desktop editing mode when I create my new sequence ! I have a 480X270 16/9 ratio.... so when I export my sequence FLV in encoder... everything is ok for audio setting ... but when I put my movie on the Internet or just playback in adobe media player, I have no sound !!!! I don't know what is the problem....
Thanks for help me !!!!

Thanks for your quick respond and support !!!!!
1- multiplexing = FLV
2- audio default : codec mpeg layer III
3 - i'm on pc
4 - I use a jcplayer for my website and in local playback i use adobe media player
I think that everything was ok in audio setting if i see my setting, but when i use a default sequence setting and I export I have sound.... when i use desktop sequence setting i lost my sound when i export !!!!!!
is very strange !!!!
Thanks again

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