Quality issues when importing still images as frames for animation

hello,
i've imported roughly 600 hand-drawn and scanned (600 dpi) images as frames for video. the images themselves are really crisp and perfectly how i want them to appear. i've imported each to play for 2 frames then move to the next. once done, i save this video file as an .avi. i then repeat the process in order to layer the video (using Calculations filter) over and over again, to fill up the screen with overlapped, layered animations. in theory, these layers should all remain crisp, just like the scanned images. i was under the impression that the standard Export... > Movie... .avi file is essentially lossless and is best for editing, however when my process is completed, the final product is muddy, grainy, and pixelated. i'm not sure where i am going wrong, as i've actually done this process before with success.
is there a way to specific the working file as "high definition" or something similar so that the quality is just as crisp as the scanned images i am using as source
any help is appreciated - i am completely self taught in video editing and it is really bugging me that the file output is not how i envisioned.
thanks,
DB

I note you specified that the images were 600 dpi.  The dot per inch are an issue only for printing a hardcopy.  For video work, the issue is the pixel dimensions and the pixel aspect ratio. If your image is 600 pixels by 400 pixels with a PAR =1.0. If your sequence is 1440 x 1080 with a pixel ratio ratio of 1.33, then the images must be the same and PrPro must scale up the image.  You can get good images from imported stills in PrPro. I prefer to rescale my images using Photoshop.  I am told that the scaling algorithm in PrPro has been improved.  But regardless, if you are scaling up, you will see degradation becauae Prpro has to interpolate between pixels to fill the video frame.

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