RS232 wont start in rtexe, but runs just fine in Debug Mode

Hi Guys,
first of all sorry for my bad english!
I have a "little" problem with my Program, especially with the builded version of my program.
As long as I am connected via my Windows machine and in the Debug Mode of my Main.vi everything works just fine, no errors and does what its intended to do.
But when i try to build my rtexe and run it as startup it wont open rs232 connection to my devices.
Any Ideas?
My installed VISA Version is 5.4.1 and I am running LV 2013 SP1.
Target is a cRIO 9082, also VISA 5.4.1 and NI RIO 13.1
Funny thing is it worked just fine a few builds back, but I did no changes on my System.

Sry for not replien earlier, I dont have access to the machine every day.
So i just added a log for the error codes and got: -1073807343
Now I am even more confused, how could that be? It says " Insufficient location information or the device or resource is not present in the system."
I am using COM7 and COM8, and as u can see in the attachment they are there! And the also work when i start the vi on my windows machine?!
Attachments:
Clipboard01.jpg ‏99 KB

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