Sending mail over VPN from Azure

Hello,
We are running a .NET website on two 2012R2 VM's behind a loadbalancer. We want to use a on-premise SMTP server for sending forms. Site to Site VPN between azure and on-premise is active.
When using telnet from both frontends we can send email with no problems at all. We use internal ip-adressess, both in azure and on-premise.
When sending email from the application we receive the error:
Message: No connection could be made because the target machine actively refused it
127.0.53.53:25
This ip-address is confusing us, I found on the ICANN website that it is a new special ip-address to mitigate the impact of name collisions in the domain name system.
However I cannot see how an azure vm or loadbalancer can have a conflicting DNS address.
Any ideas?
Regards

Finally found the issue.
The web application used a wrong hostname as sender.

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