Slow connection between outlook 2010 and Windows 2012 R2 DC

I'm having issue that affects all site users, our setup is:
1 x Exchange 2007
2 x Domain contollers, on 2008 R2 and one 2012 R2
Outlook 2010;
We have been experiencing issue's with users when they have a connection to the 2012 DC via outlook, were when they send an email it takes approx. 20 seconds for the email to be sent to the outbox. What will happen is an 'hour-glass' will appear and a bubble
appears from the outlook icon in the system tray stating that outlook is trying to establish a connection to the 2012 DC.
The users that are connected to the 2008 DC do not have this issue and the same email gets sent straight away.
We have now put in an exclusion on exchange to prevent outlook clients from connecting to the 2012 server, which has temporary resolved this issue.
as soon as we lift the exclusion the issue reappears.
I've performed PerfMon and NetMon captures of both DC's while this issue occurs but without obvious conclusion.
Has anyone come across this or able to provide with some suggestions.
thanks.
John.

Hi John,
Did you refer to Milos's suggestion and use the network monitor? Any update?
Would you please let me know whether install any third-party application (such as: some security applications) on the server 2012 r2? In addition, please type outlook.exe /safe in RUN to start Outlook in Safe Mode, then check if this issue still exists.
This will help us to confirm if the issue is caused by Outlook add-ins.
If any update, please feel free to let us know.
Best regards,
Justin Gu
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