Sudden latency! No change of buffer size or hardware. What give?

I'm trying to record a song and there's latency. I was recording two nights ago and everything was fine. The only thing that's happened between now and then is I unplugged my gear and replugged it today. The buffer size is set to 256 (and always has been as far as I know). Is it possible that the latency is due to a faulty hardware connection? I don't understand how it could be software related. Also, if the solution is to change my buffer size (which I tried and it does reduce the latency at 32) does that affect the sound quality? I find this all quite mysterious... and infuriating.

Reducing latency does not affect soundquality, but computer performance.
Buffer 256 induces pretty much latency. On my system, RME Babyface, 14.8 ms roundtrip.
And I find it quite useless to record with. So I go for buffer 64/32, or simply mute the channel I'm recording to.
And monitoring through my interface........But you didn't explain if your problem is MIdi or audio related.
With first version of LPX (10.0) I experienced som strange behaviour, too. It seems to have gone now.

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