Sync applications checkbox is disabled - cannot sync any apps

I just upgraded my iphone to 3.0. To do that I had to upgrade to itunes 8.2 first, which I could only do on a new PC. All my apps and appear fine in my itunes library. I authorised the new computer. When I try to sync, the applications are not syncing and the checkbox on the apps tab is disabled - cannot even click it on ! HELP I have wasted 3 hours already and have lost all my apps on the phone.

You can't sync applications with manually manage music and videos selected, so make sure this isn't selected. Are you syncing contacts, calendar events, and Safari bookmarks with a supported application on your computer, and are you transferring photos on your computer, or transferring iTunes music? If so, does your selected iTunes music and photos transfer to your iPhone when syncing, and are your contacts and calendar events synced with a supported application on your computer when syncing?
Have you tried disconnecting your iPhone followed by re-starting your computer, and reconnect your iPhone to iTunes after your computer has re-started to see if this makes any difference?

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