Upgrading Mac Pro (2009) from to NVIDIA Quadro 4000, what PCI slot to use ?

I am upgrading my Mac Pro (2009) from the Nvidia GT120 (stock) to the Nvidia Quadro 4000 for Mac. The GT120 was installed in the PCI Slot #1 and I would like to install the Nvidia Quadro 4000 in the PCI Slot #2, that way the Fan in the Graphics Card (Quadro 4000) will have better air circulation, is there any limitation or recommendation regarding the PCI slot to use ?, thanks a lot

I have an early Mac Pro 3,1.
The user manual I downloaded states:
Slot 1 PCI Express 2.0 16-lane
Slot 2 PCI Express 2.0 16-lane
So I'm assuming that means both slots 1 and 2 are the same and it doesn't matter which of my two graphics cards go in which slots?
Also, my second graphics card is a Quadro 4000 which is for Adobe Applications that use the CUDA engine. I don't have any monitors hooked up to it.

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