Upon starting Mac shows image of a folder with ? inside, any help appreciated, thanks.

As stated above, when I start Mac it shows a white screen with an image of a folder with a ? inside it.
I have shut it down by holding the power button then pulled the power cord waited 10mins and restarted for the same result.
Any help would be great.

Cheers for the info Allan.
I unfortunately do not have a back up.  It is in the hands of the repairer now, fingers crossed they can save my stuff.
FYI, i'll be backing up in future.

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