Usb will not open and pop up says computer will not accept it

In transferring files from a mac mini to an imac, I was able to open and load a usb.  But when I inserted the usb into the i mac, it would not open.  When I returned the usb to the mini to retrieve the files, it would not open there as well.  A pop up now appears on both screens saying it will not work with either computer.  Is it possible to retrieve the files and load them into a usb that will work?

You mean you deleted the files on the mini!? 
Did you look in the trash can for the files?
You can try to recover the files on the flash drive & the mini hd.  Stop using the mini.  Connect via firewire to your othe mac in target disk mode.
I'd try running disk utility on the flash drive.
Macintosh-HD -> Applications -> Utilities -> Disk Utility
How is the drive formated?
"Stellar Phoenix Macintosh - Mac data recovery software, recovers data from damaged, deleted, or corrupted volumes and even from initialized disks."
They have a trial version, so I guess you can see if your data can be recovered...
http://www.stellarinfo.com/mac-data-recovery.htm
"Data Rescue II is the best data recovery software on the market for recovering files from a problem hard drive. Data Rescue II works when other tools fail. Data Rescue II is also completely safe to use since it does not attempt any risky repairs to the drive while its scanning."
http://www.prosofteng.com/products/data_rescue.php
FileSalvage is an extremely powerful Macintosh application for exploring and recovering deleted files from a drive or volume.  FileSalvage is designed to restore files that have:
-- been accidentally deleted.
-- become unreadable due to media faults.
-- been stored on a drive before it was re-initialized/formatted.
http://subrosasoft.com/OSXSoftware/index.php?main_page=product_info&products_id= 1

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