Why is audio disappearing from Extracted Sound Track File?

I extracted the +Sound Track+ from a .mov file which was originally a .wmv file I'd edited and saved in QT Pro. Regarding the original movie from which I extracted the Sound Track, +Movie Properties+ indicated the +Video track+ as being +Sorenson H.263.+ The Sound Track extracted was +MPEG Layer 3.+
+MovieProperties/Audio Settings+ displayed Sound Track as a Mono audio track.
The extracted sound track appeared in a new QT Window/Player as "Untitled".
I hit play and the audio track played. I saved it as a Self-Contained Movie, hit play and it played again, no problem.
( +Movie Properties+ still displayed the +Sound Track+ Format as MPEG Layer 3/Mono)
I quit QT Pro and turned my attention to other projects in other applications.
Later, when I booted QT Pro and tried to listen to the Extracted Sound Track File, I hit play and the playhead moved, but there was no audible audio, or indication of audio in the equalizer display. It was as if the audio had disappeared. Opening +Movie Properties,+ the +Extracted Sound Track+ still appeared as MPEG layer 3/Mono and the track was still enabled.
On a hunch, I opened and played the original Movie from which I'd extracted the sound track. After that, I hit play again on the Extracted Sound Track Window/Player and voila! -- the audio miraculously reappeared and was fully audible. I don't understand. Why is this happening? I did save the Extracted Sound Track file as a self-contained movie. It doesn't make sense.
More to the point, _how does one extract a sound track and save/process it such that the extracted sound track remains intact with full playback capability, without first having to play the original movie from which it was extracted in order to make the Extracted Sound Track file playback._

Thanks RT...
The curious thing is, if I boot the computer and open the +Extracted Sound Track+ file in Quicktime, it's hit or miss. Sometimes the audio is there. Sometimes it's not. If I however, play ANY other Quicktime Movie on my computer and THEN open the +Extracted Sound Track+ File, the audio miraculously reappears. What can it be?
I've got Streamclip. Haven't used it much but I'll try your approach and export the file back to a Quicktime Format.

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Why is audio disappearing from Extracted Sound Track File?

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