Will I lose everything if I delete my last back up

I got a new 5s two weeks ago and backed it up from my old phone. Now it won't back up due to lack of space. I have deleted and turned items off and this hasn't helped. The only thing left to try is deleting my last back up from my old phone. I want to know if doing so will I lose everything and have to start from scratch or as a new phone and not from last back up!?!? Please help!!

Yes. So back up first if it's something you're considering. Right-click on the device name in iTunes and click Backup. This does not back up media (music, etc), so sync that with iTunes first if it's not already on your computer.
Matt

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