Windows 2008 R2 RADISU Server not able to authenicate Newly created AD Users on Wireless

I have a AD running on Windows 2003 R2 with Functional Level as Windows 2003 and Windows 2008 R2 serving as RADIUS Server to authenticate Wireless Users.  Suddenly since a week newly created AD users are not able to connect to Wireless where as old
users have no issue.
Could anyone suggest what would be the issue.

Hi,
Which authentication method you are using? If you are using a certificate based authenticate method, you should confirm that the clients has got
the correct certificate.
In addition, is there any errors in NPS log?

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