I have sent a file to the trash bin. When I come to empty the trash bin everything goes out except this one particular file and I get a note saying the file is 'in use' but it is not. How can I delete this file from trash?

I have a Macbook Pro with OS X Lion 10.7.2. I have sent a work file to the trash bin along with many other files. When I empty the trash this one particular file remains and I get a message that this file is still in use. I have tried returning the file to my documents, opening it, closing it and then sending it to trash and again I try to empty trash but the same message comes up - Q - How can I trash this file permanently?

First just try logging out and back in.  Whatever is holding on to it may not be running after the logout thus freeing it so you can trash it after logging back in.
You might also try Trash It!
Lastly, look at the following for suggestions:
The X Lab: Solving Trash Problems

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