Build Application That Will Run on XP & 7

If I build an application using LabVIEW 2011, will it run on XP SP2 and Windows 7 (32bit)?
Does it matter if I build my app using XP or 7?

Yes.
No.
Caveat: Windows 7 has additional folder restrictions, so if you are trying to access folders like the C:\ drive, or C:\Program Files, then your application will not work properly under Windows 7. There's a parallel discussion going on right now regarding Windows 7 compatibility: http://forums.ni.com/t5/LabVIEW/Windows-7-compatibility/td-p/1764350
The target machine needs the LabVIEW RunTime Engine.

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    ~~~~~~~~~~~~~~~~~~~~~~~~~~

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