Highest quality video for streaming

I have a series of training videos shot in HD. They will be hosted on YouTube and and Viddler and embedded in blog posts.
My source video is very high quality. I would like some guidance concerning the optimum settings when I encode the videos. My final image size will by 640 x 426.
Thanks for any assistance!
Joe Parsons

It's not very big.  I'd recommend editing and exporting at the native 1080, then using the HTML5 <video> tag for inclusion on the web page.  You can specify the size of the 'player' to those smaller dimensions, but this leaves the option for people to full screen it at the native 1080 for a better image.

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