How do I erase something from startup memory?

There's an app named "Vocal" that has a desktop counterpart program.  I tried to open the desktop program recently, only to receive the following error message:
"Vocal" can't be opened because it is from an unidentified developer.
Upon further research, I found that the developer's website was no longer functional.  I therefore uninstalled the program from my iMac and emptied the trash; I then rebooted.  Unfortunately, even though the program's deleted, I still get the error message whenever I boot up, so there's some little snippet of the program still in my iMac's startup routine.  How can I remove that from my iMac?  TIA.

That message appears when Gatekeeper detects that the developer didn't use Developer ID for the app. See > http://support.apple.com/kb/ht5290
You can open easily the app, right-clicking it and selecting "Open", or just changing its settings in System Preferences > Security and Privacy > General.
In order to stop charging the app every startup, open System Preferences > Users and Groups > Login Items, select the application and press the - button, so it won't load anymore during the start of the computer

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