I have updated my Iphone 3gs operating system but it no longer recognises my sim card, any help please?!

I've been hppily usingtoday until about half an hour ago!!!

It updated the O/S, then I went about connecting to I-Tunes, after a while it flashed up on I-Tunes "There is no SIM card installed in the I-Phone you are attempting to activate - please disconnect and try again..."
I've tried taking it out an dputting it back in several times!!
The phone now just says "no service"..
Thank you for any help!!!

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