RD Gateway prompts for authentication

Setup:
Deployment Type: 2012 RD Session based deployment
Domain level: 2012
DNS: separate internal and public domain names
Clients: Windows 8.1 ENT
Session Host: Two RDSH Collections
Broker: Single RDSB server
RD WEB: Single RDGW+RDWEB server in DMZ
SSO setup as per M$ and community KBs
Certificates: RDWEB/RDB/RDGW - Public PKI (remote.contoso.com); RDSH - Self-Signed (*.contoso.local)
Form-based authentications has been replaced with Windows Integrated and Clients successfully logon onto the Remote.Contoso.com without credential prompts.
RemoteApp Client gets configured via a GPO without any issues and Feed syncs fine.
Issue:
Upon attempt to launch RemoteApp either via Web or RemoteApp Client client receive a credential prompt from RD Gateway for authentication: *Type your user name and password to connect to Contoso RemoteApps*
Notes:
The Credential Manager shows no saved credentials for the remote.contoso.com, despite the configured RemoteApp Client. If I manually add an entry under Windows Credentials for Remote.Contoso.com with
USER1`s credentials, then there is no prompt and application launches.
Even if the credentials I enter are for USER2, even then the application is launched under the initial
USER1.

Hi,
Thanks for your comment. Sorry for late response.
Enabling the setting through the RDP file:
Alternatively, the single credential prompt setting can be manipulated from the RDP file.
1. Open the RDP file in Notepad.exe.
2. To enable the setting, the user can enter "promptcredentialonce:i:1" in the RDP file.
3. If the user wants to disable the setting, then user can enter "promptcredentialonce:i:0" in the RDP file.
More information for reference.
Single credential prompt for TS Gateway Server and Terminal Server
http://blogs.msdn.com/b/rds/archive/2007/05/04/single-credential-prompt-for-ts-gateway-server-and-terminal-server.aspx
In addition, please check the GPO setting whether you have configure the credential delegation policy for SSO. Please go through following article.
Remote Desktop Web Access single sign-on now easier to enable in Windows Server 2012
http://blogs.msdn.com/b/rds/archive/2012/06/25/remote-desktop-web-access-single-sign-on-now-easier-to-enable-in-windows-server-2012.aspx
Hope it helps!
Thanks.
Dharmesh Solanki

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