Site breaks after implementing RBLOB

Dears
I have successfully configured RBLOB for a document center site collection, however as soon as I enable blob setting on content db, the site containing documents seems distorted, and I am not able to upload any document it gives me a web.config error.
Note I first created site & then enable the rblob, hope it doesn't matter that if you create site first & then enable rblob or vice versa.
I followed below links
https://www.simple-talk.com/dotnet/.net-tools/sharepoint-2010-storing-documents-on-the-file-system-with-remote-blob-storage/
http://thesharepointfarm.com/2011/03/enabling-rbs-on-multiple-content-databases/

Sorry guys for replying late, I checked the db permission for app pool exists, still the same error.
Below is the log from event viewer while trying to upload file of any size
Event code: 3005
Event message: An unhandled exception has occurred.
Event time: 1/7/2015 12:41:35 PM
Event time (UTC): 1/7/2015 8:41:35 AM
Event ID: 1608b255b64f44618f95b111821b685b
Event sequence: 8128
Event occurrence: 258
Event detail code: 0
Application information:
    Application domain: /LM/W3SVC/489769728/ROOT-1-130650585599244699
    Trust level: Full
    Application Virtual Path: /
    Application Path: C:\inetpub\wwwroot\wss\VirtualDirectories\80\
    Machine name: RAECVSPAPP
Process information:
    Process ID: 9468
    Process name: w3wp.exe
    Account name: RURAL\sp_pool2013
Exception information:
    Exception type: SPException
    Exception message: The URL 'test/Read me.txt' is invalid.  It may refer to a nonexistent file or folder, or refer to a valid file or folder that is not in the current Web.
   at Microsoft.SharePoint.SPGlobal.HandleComException(COMException comEx)
   at Microsoft.SharePoint.Library.SPRequest.PutFile(String bstrUrl, String bstrWebRelativeUrl, Object punkFile, Int64 cbFile, Object punkSPFileMgr, Object punkFFM, Int64 PutFileOpt, String bstrCreatedBy, String bstrModifiedBy, Int32 iCreatedByID,
Int32 iModifiedByID, Object varTimeCreated, Object varTimeLastModified, Object varProperties, String bstrCheckinComment, String bstrLockIdMatch, String bstEtagToMatch, Int32 lockType, String lockId, Int32 minutes, Int32 fRefreshLock, Int32 bValidateReqFields,
Guid gNewDocId, SPFileSaveParams sfsp, SPFileInfo& pFileProps, UInt32& pdwVirusCheckStatus, String& pVirusCheckMessage, String& pEtagReturn, Byte& piLevel, Int32& pbIgnoredReqProps)
   at Microsoft.SharePoint.SPFileCollection.AddStreamOrBytesInternal(String urlOfFile, Stream file, Int64 fileSizeToSave, SPFileStreamManager spmgr, PutFileOpt fileOpt, String createdBy, String modifiedBy, Int32 createdByID, Int32 modifiedByID, DateTime
timeCreated, DateTime timeLastModified, Object varProperties, String checkInComment, Stream formatMetadata, String lockIdMatch, String etagToMatch, SPLockType lockType, String lockId, TimeSpan lockTimeout, Boolean validateRequiredFields, SPVirusCheckStatus&
virusCheckStatus, String& virusCheckMessage, String& etagNew, Boolean& ignoredRequiredProps)
   at Microsoft.SharePoint.SPFileCollection.Add(String urlOfFile, Stream file, SPFileCollectionAddParameters parameters)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadPage.UploadFile(HttpPostedFile requestFile, String& leafName, SPVirusCheckStatus& checkStatus, String& virusMessage)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadPage.UploadFile(HttpPostedFile requestFile)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadPage.OnSubmit(Object o, EventArgs e)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadExPage.OnSubmit(Object o, EventArgs e)
   at System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint)
<nativehr>0x81020030</nativehr><nativestack></nativestack>The URL 'test/Read me.txt' is invalid.  It may refer to a nonexistent file or folder, or refer to a valid file or folder that is not in the current Web.
   at Microsoft.SharePoint.Library.SPRequestInternalClass.PutFile(String bstrUrl, String bstrWebRelativeUrl, Object punkFile, Int64 cbFile, Object punkSPFileMgr, Object punkFFM, Int64 PutFileOpt, String bstrCreatedBy, String bstrModifiedBy, Int32
iCreatedByID, Int32 iModifiedByID, Object varTimeCreated, Object varTimeLastModified, Object varProperties, String bstrCheckinComment, String bstrLockIdMatch, String bstEtagToMatch, Int32 lockType, String lockId, Int32 minutes, Int32 fRefreshLock, Int32 bValidateReqFields,
Guid gNewDocId, SPFileSaveParams sfsp, SPFileInfo& pFileProps, UInt32& pdwVirusCheckStatus, String& pVirusCheckMessage, String& pEtagReturn, Byte& piLevel, Int32& pbIgnoredReqProps)
   at Microsoft.SharePoint.Library.SPRequest.PutFile(String bstrUrl, String bstrWebRelativeUrl, Object punkFile, Int64 cbFile, Object punkSPFileMgr, Object punkFFM, Int64 PutFileOpt, String bstrCreatedBy, String bstrModifiedBy, Int32 iCreatedByID,
Int32 iModifiedByID, Object varTimeCreated, Object varTimeLastModified, Object varProperties, String bstrCheckinComment, String bstrLockIdMatch, String bstEtagToMatch, Int32 lockType, String lockId, Int32 minutes, Int32 fRefreshLock, Int32 bValidateReqFields,
Guid gNewDocId, SPFileSaveParams sfsp, SPFileInfo& pFileProps, UInt32& pdwVirusCheckStatus, String& pVirusCheckMessage, String& pEtagReturn, Byte& piLevel, Int32& pbIgnoredReqProps)
Request information:
    Request URL:
http://raecvspapp/_layouts/15/UploadEx.aspx?List={59E76088-CFB2-4FA8-AE49-8B6ACC95FB63}&RootFolder=&IsDlg=1
    Request path: /_layouts/15/UploadEx.aspx
    User host address: ::1
    User: 0#.w|rural\sp_admin2013
    Is authenticated: True
    Authentication Type: Federation
    Thread account name: RURAL\sp_pool2013
Thread information:
    Thread ID: 36
    Thread account name: RURAL\sp_pool2013
    Is impersonating: False
    Stack trace:    at Microsoft.SharePoint.SPGlobal.HandleComException(COMException comEx)
   at Microsoft.SharePoint.Library.SPRequest.PutFile(String bstrUrl, String bstrWebRelativeUrl, Object punkFile, Int64 cbFile, Object punkSPFileMgr, Object punkFFM, Int64 PutFileOpt, String bstrCreatedBy, String bstrModifiedBy, Int32 iCreatedByID,
Int32 iModifiedByID, Object varTimeCreated, Object varTimeLastModified, Object varProperties, String bstrCheckinComment, String bstrLockIdMatch, String bstEtagToMatch, Int32 lockType, String lockId, Int32 minutes, Int32 fRefreshLock, Int32 bValidateReqFields,
Guid gNewDocId, SPFileSaveParams sfsp, SPFileInfo& pFileProps, UInt32& pdwVirusCheckStatus, String& pVirusCheckMessage, String& pEtagReturn, Byte& piLevel, Int32& pbIgnoredReqProps)
   at Microsoft.SharePoint.SPFileCollection.AddStreamOrBytesInternal(String urlOfFile, Stream file, Int64 fileSizeToSave, SPFileStreamManager spmgr, PutFileOpt fileOpt, String createdBy, String modifiedBy, Int32 createdByID, Int32 modifiedByID, DateTime
timeCreated, DateTime timeLastModified, Object varProperties, String checkInComment, Stream formatMetadata, String lockIdMatch, String etagToMatch, SPLockType lockType, String lockId, TimeSpan lockTimeout, Boolean validateRequiredFields, SPVirusCheckStatus&
virusCheckStatus, String& virusCheckMessage, String& etagNew, Boolean& ignoredRequiredProps)
   at Microsoft.SharePoint.SPFileCollection.Add(String urlOfFile, Stream file, SPFileCollectionAddParameters parameters)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadPage.UploadFile(HttpPostedFile requestFile, String& leafName, SPVirusCheckStatus& checkStatus, String& virusMessage)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadPage.UploadFile(HttpPostedFile requestFile)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadPage.OnSubmit(Object o, EventArgs e)
   at Microsoft.Office.RecordsManagement.PolicyFeatures.ApplicationPages.UploadExPage.OnSubmit(Object o, EventArgs e)
   at System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint)
Custom event details:

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