Histogram mean and median in Photoshop are different than in MATLAB

Hi guys. I'm currently using Photoshop CS3 on my PC and I've been tasked to do some image analysis research. I used Photoshop to analyze a pool of 100 files to find the mean and median of their (intensity/gray) histograms. (Using the im2gray function in MATLAB)
The problem is, though, that when I analyze these same files in MATLAB with the mean (mean2), median (median(I(:)) I am coming up with different numbers. Some of the means/medians in MATLAB are as high as 15 (scale: uint8 (1-256)) away from the results in Photoshop.
I am trying to find the source of this problem, but I cannot put my finger on it. I'm thinking photoshop and MATLAB just process images differently or something but I would like to know the root cause of the problem.
If anyone can help here I'd appreciate it much!

I don't use Mathlab so this is just a shot in the dark but you might want to try going to the Edit menu and choosing Assign Profile. In the dialog choose 'Don't Color Manage This Document'.
I would think that would sent out values to Mathlab not adjusted to any profile. If that doesn't work you might try the sGray profile or one of the Gamma.
As I understand it Dot Gain is how the ink spreads on paper when screened and printed. It has to do with how much ink is layed down by the press and the paper stock.

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