If I delete songs off of a playlist on my iPhone 4S, will the songs delete off of the iTunes playlist when I sync?

So basically, I have a LOT of music. Like, over 26,000 songs a lot. I am now in the process of going through all of it, song by song, and I have been making playlists on iTunes and syncing them to my phone. Now, I know I can delete the songs manually off of my iPhone, but will they delete off of iTunes as well once I sync back to the computer? I'm worried that all of my work will be for naught. And if there is a way to delete the songs off of iTunes, other than manually selecting and deleting, please let me know. Thank you!

B2Veil,
The best way to ensure the content gets removed from your iPhone is to back it up and then Erase All Content and Settings.   See the article belows below for more info:
iOS: How to back up and restore your content
iOS: Understanding 'Erase All Content and Settings'
You can remove all settings and information from your iPhone, iPad, or iPod touch using "Erase All Content and Settings" in Settings > General > Reset.
I hope this information helps ....
Have a great day!
- Judy

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