How can I improve the response time of the user interface?

I'm after some tips on how to improve the response time to mouse clicks on a VI front panel.
I have data acquistion application which used to run fine, but after spending a couple of weeks making a whole bunch of changes to it I find that the user interface has become a bit sluggish.
My main GUI VI has a while loop running 16 times a second, updating some waveform charts and polling about a dozen buttons on the front panel.
There is sometimes a delay (variable, but up to 2 seconds sometimes) from when I click on a button to when it becomes depressed. I have wired the iteration terminal of the while loop to an indicator on the front panel and can see that the while loop is ticking over during the delayed response to the mouse click, so I know that the problem is not that the whole program is running slow, just the response to mouse clicks.
Also, just for debugging purposes, I have indicators of the iterations of all the main while loops in my program on the front panel, so I can see that there are no loops running abnormally fast either.
One thing I've tried is to turn off multi-threading, and this does seem to work - the response to mouse clicks is much faster. However, it has the side effect of making the main GUI while loop run less evenly. I was trying to get a fairly smooth waveform scrolling across the screen, and when multi-threading is off it gets a bit jerky.
Any other suggestion welcome..
(I am using LabVIEW 7.1, Windows 2000).
Regards,
Mark.

Hi Altenbach,
Thanks for your reply. In answer to your questions:
I am doing both DAQ board and serial data acquisition. I am using NIDAQ traditional for the DAQ board, and VISA for the serial. I have other similar versions of this program that do only DAQ board, or only serial, and these work fine. It was only when I combined them both into the same program that I ran into problems.
The multiple while loops are actually in separate VIs. I have one VI that acquires data from the DAQ card, another VI that acquires data from the serial port, another VI that processes the data and saves to file, and another VI, the GUI VI, that displays the data in graphs and charts. The data is transferred betwen the VIs via LV2 globals.
The GUI VI is a bit more complicated than I first mentioned. It has tab control, with 4 waveform charts on one page, 4 waveform graphs on another page, and 3 waveform graphs on another page. The charts have a history length of 2560, and 16 data points are added 16 times a second. The wavefom graphs are only updated once per minute.
I don't use the value property at all, but I do use lots of property nodes for changing the properties of the graphs and charts e.g. changing plot colours, Y scale range etc. There is only one local variable (for the Tab control). All the graphs and charts have data wired directly to their terminals.
I haven't done any profiling yet.
I am building arrays in uninitialised shift registers, but this is all well under control. As the experiement goes on, more data is collected and stored, and so the memory usage does gradually increase, but only to the extent that I would expect.
The CPU usage is 100%, but I thought this was always the case when using NIDAQ with DAQ cards. Or am I wrong about this? (As a side note, I am using NIDAQ traditional, but would NIDAQmx be better?)
Execution priority of the GUI vi (and all the other VIs for that matter) is set to normal.
The program is a bit large to post here, and I'm not sure if my company would be happy for me to publicise it anyway, so I suspect that this is turning into one of those questions that are going to be impossible to answer.
Just as a more general question, why would turning off multi-threading improve the user interface response?
Thanks,
Mark.

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