Interruption of service in case of reapplying permissions

Hello everyone. I've a question:
Have a shared folder on 2008 R2 server. In its NTFS permissions i've got 2 domain groups + localadmins + SYSTEM account. Share is rather old, and subfolders in in have different owners and permissions. I'm trying to make a replica of this folder
using DFS. It doesn't work. Also, some of the files & folders in this share have no permissions for local or even domain admins to view\copy it. I'm thinking of replacing owner of share (and subfolders) to local server administrators, but afraid what this
share will be unaccessible during or after reapplying permission. Is it safe, for example to change owners of share and subfolders (will old permission stay?) and then re-add group of local admins & SYSTEM account modify permission, so DFSR can work after
all?

Hi,
The owner has an implied right to allow or deny other users permission to use the object, and this right cannot be withdrawn. Among other permissions, owners can give other users permission to Change Permissions (WRITE_DAC).
If you only add domain admins to view\copy the shared folder, old permissions will not change.
How Owners Are Assigned and Changed
http://technet.microsoft.com/en-us/library/cc961992.aspx
Regards,
Mandy
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