Sites Intermittently Not Responding After Service Pack 2

Got a strange intermittent issue occurring after we upgraded our SharePoint 2010 farm to Service Pack 2.
The upgrade was successful on all of our servers with no errors and generally performance is a little quicker as should be expected. However we have an intermittent issue where some site collections within the main web application become unresponsive.
We have 2 front end servers sitting behind a network load balancer. 
All sites have a dedicated content database.
The issue occurs on either front end server.
An app pool recycle fixes the issue until it occurs again, however we can't go recycling app pools constantly during business hours as we have around 3000 users connecting to our SharePoint portal.
ULS logs and event logs don't show anything abnormal really (however just dialled up the logging to verbose to look into it further).
Any suggestions?

Hi,
By default, the application pool is set to recycle at a specific time automatically in IIS.
So the issue you met may be due to the application pool cannot be recycled automatically or the recycle was crashed.
I recommend to check IIS log for SharePoint web application and
System event logs on the IIS server for the corresponding web application to see the error message.
http://blog.bugrapostaci.com/2012/04/12/how-to-collect-iis-logs-for-a-sharepoint-web-application/
http://blogs.msdn.com/b/benjaminperkins/archive/2013/07/01/troubleshooting-badly-behaving-iis-application-pools.aspx
Thanks,
Victoria
Forum Support
Please remember to mark the replies as answers if they help and unmark them if they provide no help. If you have feedback for TechNet Subscriber Support, contact
[email protected]
Victoria Xia
TechNet Community Support

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