Problems accesing files in Mac Mini for Windows users.

We have a Mac Mini that we use as a data server in our network. Our main server is Windows and most of the users are Windows users.
At first, we had dificulties for connecting this Windows users to the server, but finally we did it and they have been working good accesing the files on the server, until last saturday when we run an update on the server.
All the privileges for files and folders were distorted and the users that acces the server with Windows 7 and 8, can't open some files and can't modify others (there is a warning that says that the file is in use by another user and can only be read).
We already re asigned the privileges for each user and folders, but we are still having problems with users that work with Windows 7 and 8 because they can not open any file. Windows XP users do not have this problem and they are working fine.
Can someone help me with this issue?

Hello,
Since XP works & Win7&8 do not, I wonder if disabling SMBv2 & above might fix it...
http://support.microsoft.com/kb/2696547

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