Server Error in '/' Application - The resource cannot be found

Using VS 2008 with Framework 3.5, I built a web-service that runs perfectly on the development PC.  When I build and run the web service, it runs in a virtual port as "ASP.Net Development Server Port 49259".  All the methods will
invoke no problem.  However, if I try bring up this web-service asmx page on another PC or the mobile app that is cradled to the development PC via USB, I get: Server Error in '/' Application. The resource cannot be found.  HTTP 404... 
This app ran just fine back in October13 but I opened for further development over the weekend and I cannot work because of this.  I have updated PC and VS with latest updates (problem before & after updates).  Using 3.5 because I am using
Windows Mobile 5.0 Clients.  I have searched the forum universe for the solution and have found many people with this error but with other platforms.
I am using the DefaultApplicationPool and I made sure that the local security has READ access on the web service file folders. I hope this is something simple that I am missing.
And help - grateful!

Hello,
Thank you for your post.
I am afraid that the issue is out of support range of VS General Question forum which mainly discusses
the usage of Visual Studio IDE such as WPF & SL designer, Visual Studio Guidance Automation Toolkit, Developer Documentation and Help System
and Visual Studio Editor.
If you are working with ASP.NET Web Application, I suggest that you can consult your issue on ASP.NET
WCF, ASMX and other Web Services forum for better solution and support.
Best regards,
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