Algorithm to find the area and color of the objects in an image

Question

Hello everyone,
I have some problems in finding the segmented objects once i find the threshold , i want to find the area of the objects in the image . for example i have some lymphocytes in the image and i need to seperate them from the background by labelling them. can i seperate them by using the colour as well . please give some idea .
my code till now is:
int data = -1;
try {
System.out.println("Is the input a FloatImage ?");
m_fi_in = (FloatImage)inputData[0];
data = 0;
catch (ClassCastException cce) {
System.out.println("Not a FloatImage");
data = -1;
try {
System.out.println("Is the input a Volume ?");
m_vol_in = (Volume)inputData[0];
data = 1;
catch (ClassCastException cce2) {
System.out.println("Not a Volume");
data = -1;
System.out.println(data);
// For the FloatImage
if (data == 0) {
// For the Volume
else if(data == 1) {
System.out.println("Entering Volume for segmentation....");
// Get target dimensions
int depth = m_vol_in.getDepth();
int height = m_vol_in.getHeight();
int width = m_vol_in.getWidth();
int region = 1;
// Create a label structure
// Consists of Image and region info
m_label = new Label(m_vol_in);
if(m_label == null) {
System.out.println("Label could not be created");
return false;
// apply the threshold set by user
float threshold = m_threshold.getValue();
float maxi = m_threshold.getMaximum();
float mini = m_threshold.getMinimum();
// label the center of the volume in the region part of label as region 1
m_label.setRegion(m_start_z.getValue(), m_start_y.getValue(), m_start_x.getValue(), region);
System.out.println("Set starting voxel ("+m_start_z.getValue()+","+m_start_y.getValue()+","+m_start_x.getValue()+" to "+region);
// Now process 8 partial volumina starting from a given point in the yz-plane in positive and then negative x directions
// check the neighbours if they are already segmented and set the region in the target volume accordingly
// do so for the whole yz-plane
int x,y,z;
int dir = 0; // the direction of the search. Only initialised here !
System.out.println("Processing Volume in z++");
for (z = m_start_z.getValue(); z < depth-1; z++) {
for (y = m_start_y.getValue(); y < height-1; y++) {
dir = 1;
for (x = m_start_x.getValue(); x < width-1; x++) {
checkRegion(m_label, z, y, x, threshold, region, dir);
dir = -1;
for (x = m_start_x.getValue(); x > 0; x--) {
checkRegion(m_label, z, y, x, threshold, region, dir);
for (y = m_start_y.getValue(); y > 0; y--) {
dir = 1;
for (x = m_start_x.getValue(); x < width-1; x++) {
checkRegion(m_label, z, y, x, threshold, region, dir);
dir = -1;
for (x = m_start_x.getValue(); x > 0; x--) {
checkRegion(m_label, z, y, x, threshold, region, dir);
System.out.println("Processing Volume in z--");
for (z = m_start_z.getValue(); z > 0 ; z--) {
for (y = m_start_y.getValue(); y < height-1; y++) {
dir = 1;
for (x = m_start_x.getValue(); x < width-1; x++) {
checkRegion(m_label, z, y, x, threshold, region, dir);
dir = -1;
for (x = m_start_x.getValue(); x > 0; x--) {
checkRegion(m_label, z, y, x, threshold, region, dir);
for (y = m_start_y.getValue(); y > 0; y--) {
dir = 1;
for (x = m_start_x.getValue(); x < width-1; x++) {
checkRegion(m_label, z, y, x, threshold, region, dir);
dir = -1;
for (x = m_start_x.getValue(); x > 0; x--) {
checkRegion(m_label, z, y, x, threshold, region, dir);
// Apply result to output
outputData[0] = m_label;
else {
return false;
}

Answer 1

search for cluster analysis
spieler