AnyConnect client 3.1.04063 Windows 7 x64 users cannot make ssl connection

Over the past week several of my users have suddenly found they cannot connect with a previously working client.  After the login banner is accepted they all get an error message "The certificate on the secure gateway is invalid.  A VPN connection will not be established."  Then another message "AnyConnect was not able to establish a conenction to the specified secure gateway.  Please try connecting again."  On the ASA 5540 logs I see successful authentication and then the device is trying to establish a ssl session which is denied and then connection is terminated.
I have verfied that the ssl certificates are valid and are installed in the trusted root certificates location.  I have checked that ICS is disabled.  I have checked that the vpn adapter display name is correct.
Does anyone have any ideas?

We are not using a self signed cert.  We have a cert issued by the DoD.  It seems like a user who had previously connected and is on Windows 7 x64 will not be able to connect.  Users who have never connected and browse to the site will be able to successfully connect.
Additional information:  I have cleared all DoD related certs and the server cert from the certmgr.msc on an affected Windows box.  Uninstalled the AnyConnect application and all remnant files.  Cleared SSL cache on both IE and Firefox browsers.  I rebooted then tried connecting via the web address but am receiving the same issue.

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    crypto ikev2 remote-access trustpoint asa_cert_trustpoint
    telnet timeout 5
    ssh LAN-10-0-1-x 255.255.255.0 Internal
    ssh xxx.xxx.xxx.xxx 255.255.255.255 External
    ssh xxx.xxx.xxx.xxx 255.255.255.255 External
    ssh timeout 5
    ssh version 2
    console timeout 0
    no vpn-addr-assign aaa
    no ipv6-vpn-addr-assign aaa
    no ipv6-vpn-addr-assign local
    dhcpd dns 75.153.176.9 75.153.176.1
    dhcpd domain ingo.local
    dhcpd option 3 ip 10.0.1.254
    dhcpd address 10.0.1.50-10.0.1.81 Internal
    dhcpd enable Internal
    threat-detection basic-threat
    threat-detection scanning-threat shun except ip-address LAN-10-0-1-x 255.255.255.0
    threat-detection statistics access-list
    threat-detection statistics tcp-intercept rate-interval 30 burst-rate 400 average-rate 200
    dynamic-filter use-database
    dynamic-filter enable interface Internal
    dynamic-filter enable interface External
    dynamic-filter drop blacklist interface Internal
    dynamic-filter drop blacklist interface External
    ntp server 128.233.3.101 source External
    ntp server 128.233.3.100 source External prefer
    ntp server 204.152.184.72 source External
    ntp server 192.6.38.127 source External
    ssl encryption aes256-sha1 aes128-sha1 3des-sha1
    ssl trust-point asa_cert_trustpoint External
    webvpn
    port 44433
    enable External
    dtls port 44433
    anyconnect image disk0:/anyconnect-win-3.1.02026-k9.pkg 1
    anyconnect profiles profile1 disk0:/profile1.xml
    anyconnect enable
    smart-tunnel list SmartTunnelList1 mstsc mstsc.exe platform windows
    smart-tunnel list SmartTunnelList1 putty putty.exe platform windows
    group-policy DfltGrpPolicy attributes
    vpn-tunnel-protocol ikev1 ikev2 l2tp-ipsec ssl-client ssl-clientless
    webvpn
      anyconnect profiles value profile1 type user
    username write.ingo password ... encrypted
    username ingo password ... encrypted privilege 15
    username tom.tucker password ... encrypted
    class-map TCP
    match port tcp range 1 65535
    class-map type regex match-any BlockFacebook
    match regex BlockFacebook
    class-map type inspect http match-all BlockDomains
    match request header host regex class BlockFacebook
    class-map inspection_default
    match default-inspection-traffic
    policy-map type inspect dns preset_dns_map
    parameters
      message-length maximum client auto
      message-length maximum 1500
      id-randomization
    policy-map TCP
    class TCP
      set connection conn-max 1000 embryonic-conn-max 1000 per-client-max 250 per-client-embryonic-max 250
      set connection timeout dcd
      set connection advanced-options Normalizer
      set connection decrement-ttl
    policy-map type inspect http HTTP
    parameters
      protocol-violation action drop-connection log
    class BlockDomains
    policy-map global_policy
    class inspection_default
      inspect ftp
      inspect h323 h225
      inspect h323 ras
      inspect rsh
      inspect rtsp
      inspect esmtp
      inspect sqlnet
      inspect skinny 
      inspect sunrpc
      inspect xdmcp
      inspect sip 
      inspect netbios
      inspect tftp
      inspect ip-options
      inspect dns preset_dns_map dynamic-filter-snoop
      inspect http HTTP
    service-policy global_policy global
    service-policy TCP interface External
    smtp-server 199.185.220.249
    privilege cmd level 3 mode exec command perfmon
    privilege cmd level 3 mode exec command ping
    privilege cmd level 3 mode exec command who
    privilege cmd level 3 mode exec command logging
    privilege cmd level 3 mode exec command failover
    privilege cmd level 3 mode exec command vpn-sessiondb
    privilege cmd level 3 mode exec command packet-tracer
    privilege show level 5 mode exec command import
    privilege show level 5 mode exec command running-config
    privilege show level 3 mode exec command reload
    privilege show level 3 mode exec command mode
    privilege show level 3 mode exec command firewall
    privilege show level 3 mode exec command asp
    privilege show level 3 mode exec command cpu
    privilege show level 3 mode exec command interface
    privilege show level 3 mode exec command clock
    privilege show level 3 mode exec command dns-hosts
    privilege show level 3 mode exec command access-list
    privilege show level 3 mode exec command logging
    privilege show level 3 mode exec command vlan
    privilege show level 3 mode exec command ip
    privilege show level 3 mode exec command failover
    privilege show level 3 mode exec command asdm
    privilege show level 3 mode exec command arp
    privilege show level 3 mode exec command ipv6
    privilege show level 3 mode exec command route
    privilege show level 3 mode exec command ospf
    privilege show level 3 mode exec command aaa-server
    privilege show level 3 mode exec command aaa
    privilege show level 3 mode exec command eigrp
    privilege show level 3 mode exec command crypto
    privilege show level 3 mode exec command ssh
    privilege show level 3 mode exec command vpn-sessiondb
    privilege show level 3 mode exec command vpnclient
    privilege show level 3 mode exec command vpn
    privilege show level 3 mode exec command dhcpd
    privilege show level 3 mode exec command blocks
    privilege show level 3 mode exec command wccp
    privilege show level 3 mode exec command dynamic-filter
    privilege show level 3 mode exec command webvpn
    privilege show level 3 mode exec command service-policy
    privilege show level 3 mode exec command module
    privilege show level 3 mode exec command uauth
    privilege show level 3 mode exec command compression
    privilege show level 3 mode configure command interface
    privilege show level 3 mode configure command clock
    privilege show level 3 mode configure command access-list
    privilege show level 3 mode configure command logging
    privilege show level 3 mode configure command ip
    privilege show level 3 mode configure command failover
    privilege show level 5 mode configure command asdm
    privilege show level 3 mode configure command arp
    privilege show level 3 mode configure command route
    privilege show level 3 mode configure command aaa-server
    privilege show level 3 mode configure command aaa
    privilege show level 3 mode configure command crypto
    privilege show level 3 mode configure command ssh
    privilege show level 3 mode configure command dhcpd
    privilege show level 5 mode configure command privilege
    privilege clear level 3 mode exec command dns-hosts
    privilege clear level 3 mode exec command logging
    privilege clear level 3 mode exec command arp
    privilege clear level 3 mode exec command aaa-server
    privilege clear level 3 mode exec command crypto
    privilege clear level 3 mode exec command dynamic-filter
    privilege cmd level 3 mode configure command failover
    privilege clear level 3 mode configure command logging
    privilege clear level 3 mode configure command arp
    privilege clear level 3 mode configure command crypto
    privilege clear level 3 mode configure command aaa-server
    prompt hostname context
    no call-home reporting anonymous
    call-home
    profile CiscoTAC-1
      no active
      destination address http https://tools.cisco.com/its/service/oddce/services/DDCEService
      destination address email [email protected]
      destination transport-method http
      subscribe-to-alert-group diagnostic
      subscribe-to-alert-group environment
      subscribe-to-alert-group inventory periodic monthly
      subscribe-to-alert-group configuration periodic monthly
      subscribe-to-alert-group telemetry periodic daily
    Cryptochecksum:41a021a28f73c647a2f550ba932bed1a
    : end
    Many thanks,
    Ingo

    Hi Jose,
    here is what I got now:
    ASA(config)# sh run | begin tunnel-group
    tunnel-group DefaultWEBVPNGroup general-attributes
    address-pool VPNPool
    authorization-required
    and DAP debugging still the same:
    ASA(config)# DAP_TRACE: DAP_open: CDC45080
    DAP_TRACE: Username: tom.tucker, aaa.cisco.grouppolicy = DfltGrpPolicy
    DAP_TRACE: Username: tom.tucker, aaa.cisco.username = tom.tucker
    DAP_TRACE: Username: tom.tucker, aaa.cisco.username1 = tom.tucker
    DAP_TRACE: Username: tom.tucker, aaa.cisco.username2 =
    DAP_TRACE: Username: tom.tucker, aaa.cisco.tunnelgroup = DefaultWEBVPNGroup
    DAP_TRACE: Username: tom.tucker, DAP_add_SCEP: scep required = [FALSE]
    DAP_TRACE: Username: tom.tucker, DAP_add_AC:
    endpoint.anyconnect.clientversion="3.1.02026";
    endpoint.anyconnect.platform="win";
    DAP_TRACE: Username: tom.tucker, dap_aggregate_attr: rec_count = 1
    DAP_TRACE: Username: tom.tucker, Selected DAPs: DfltAccessPolicy
    DAP_TRACE: Username: tom.tucker, DAP_close: CDC45080
    Unfortunately, it still doesn't work. Hmmm.. maybe a wipe of the config and starting from scratch can help?
    Thanks,
    Ingo

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