Copy Queue Length - All of a sudden one server having communication issues

We have 4 servers in a DAG (3 at site A and 1 at site B).
Of the three servers at site A two of them always show 0 copy queue length.  Recently one of the servers started to show a back log and we are seeing the following in the event viewer.  We see this error when this problem server connects to either
of the other two in the same physical site.
The log copier was unable to communicate with server 'ABC1'. The copy of database 'DB2\ABC1' is in a disconnected state. The communication error was: An error occurred while communicating with server
'ABC1'. Error: Unable to read data from the transport connection: An existing connection was forcibly closed by the remote host. The copier will automatically retry after a short delay.
At night the queue goes back to 0 and we start over again.  Currently the problem server only has passive copies, we moved of active just in case.
I have tried using the MAPI network to replicate (Different physical NICs and switches), that was just worse.  Also tried deactivating the primary NIC in the team and using the secondary that is connected to a different core switch.
Any ideas? 

Hi,
Basic on your post, I understand that one DAG member always show 0 copy queue length with error “Unable to read data from the transport connection: An existing connection was forcibly closed by the remote host. The copier will automatically retry after a
short delay”.
If I misunderstand your concern, please do not hesitate to let me know.
Please run below command to double check the connectivity between server:
1. Use netsh int tcp show global.
2. Use netsh int tcp to set global autotuninglevel=disabled.
3. Use netsh int tcp to set global chimney=disabled.
4. Use netsh int tcp to set global rss=disabled.
Meanwhile, follow below steps:
1. Please use the Get-DatabaseAvailabilityGroupNetwork cmdlet to check if DAG network is ok.
2. Run the Update-MailboxDatabaseCopy -Identity xx cmdlet to seed a copy of a database.
3. Restart the Microsoft Exchange Replication service.
4. Please ensure that port 64327 is open.
Thanks
Please remember to mark the replies as answers if they help, and unmark the answers if they provide no help. If you have feedback for TechNet Support, contact [email protected]
Allen Wang
TechNet Community Support

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