I can't send mail since updating Mavericks

I have updated Mavericks to version 10.9.2 and now I can't send emails. I can recieve them and read them but not reply. I have used connection doctor and it has come back and said that all is well. Please can anybody help?

From the Mail menu bar, select
Window ▹ Connection Doctor
Click the Show Detail button. A drawer opens. Try to send a message and post the text that appears. Anonymize any personal information before posting.

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